Hyperthermia induced changes in serum I-CTP and MMP-1
Journal of Bone and Mineral metabolism
Takeuchi Akira, Shimizu Masao, and five authors
Department of Thermotherapy, Luke Hospital, Clinical Laboratory, Saitama Medical School, Department of Health Care Unit, Tokyo Teishin Hospital
Purpose: Serum level of I-CPT, one of the degradation products of type I collagen, has been known to rise in patients with osteoporosis or bone metastasis, Recently, I-CPT has received another attention as the important marker for new therapy by matrix metalloproteinase-inhibitor (MMPI) for patients with metastasis. In almost all cancer patients undergoing Whole body hyperthermia using infrared irradiation, we have noted a rapid rise of serum I-CPT, Furthermore, we monitored changes in I-CPT and MMP-1 (Interstitial collagenase) by heat.
Subjects: We evaluated serum I-CPT and MMP-1, before and immediately after induction of Whole body hyperthermia at 41.0 – 41.8℃-60min, as well as 3 hours and one week after in 25 advanced cancer patients without bone metastasis,
Results: Mean of I-CPT elevated significantly to abnormal level, immediately after heating, and MMP-1 level significantly decreased at 3 hours after heating and returned to prior level one week after.
Conclusion: Since this phenomenon is not associated with any other metabolic changes indicating bone resorption, there is a possibility that an increase in serum I-CPT level alone may account for degradation of type-I collagen in connective tissues other than bones. So it is well-known that newly-synthesized collagen molecules undergo a structural alteration of the protein if human collagen is heated at 40℃ or higher.
